- Title
- Expression of tyrosine hydroxylase increases the resistance of human neuroblastoma cells to oxidative insults
- Creator
- Franco, Jeferson L.; Posser, Thais; Gordon, Sarah L.; Bobrovskaya, Larisa; Schneider, Jennifer J.; Farina, Marcelo; Dafre, Alcir L.; Dickson, Phillip W.; Dunkley, Peter R.
- Relation
- Toxicological Sciences Vol. 113, Issue 1, p. 150-157
- Publisher Link
- http://dx.doi.org/10.1093/toxsci/kfp245
- Publisher
- Oxford University Press
- Resource Type
- journal article
- Date
- 2010
- Description
- In this study, we demonstrate that human neuroblastoma SHSY5Y cells transfected with human tyrosine hydroxylase isoform 1 (SH + TH cells) were substantially more resistant to cell death induced by hydrogen peroxide and 6-hydroxydopamine when compared to wild-type SH-SY5Y cells (SH cells). SH + TH cells exhibit increased levels of dopamine (DA) compared to SH cells. Incubation with hydrogen peroxide or 6-hydroxydopamine (10–100mM) for 24 h caused a significant reduction in cell viability and increased apoptosis in both cell types. However, these effects were significantly reduced in the SH 1 TH cells when compared to the SH cells. The SH + TH cells showed an improved ability to detoxify peroxide, which correlated with an increase in glutathione peroxidase and glutathione reductase activities, while catalase activity was unchanged. Our data suggest that a preconditioning like mechanism linked to higher DA levels increased the resistance of SH + TH cells against oxidative insults, which is at least in part related to an augmentation in the activity of glutathione-related antioxidant enzymes.
- Subject
- tyrosine hydroxylase; dopamine; oxidative stress; gluthathione peroxidase; glutathione reductase
- Identifier
- http://hdl.handle.net/1959.13/922500
- Identifier
- uon:9573
- Identifier
- ISSN:1096-6080
- Language
- eng
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